Flowjo internship
The answer to this question depends primarily on your goal (enrichment vs. Therefore, we recommend that you bring 25-50% more cells to the sorter than you would need if the actual yield were 100% (based on the abundance of your target cells). However, the actual yield is usually 75-95% of this theoretical yield, due to abort rates (caused by sort conflicts) and also the quality of your sample. In theory you would need to run 10 million cells through the sorter (10 million x 20% = 2 million). Do you want stringent purification or enrichment with great recovery?Īll of these parameters influence the yield and purity of cell sorting.įor example: Let's say the subset you want sorted is 20% of the total and you need 2 million cells for your experiment.What is the approximate percentage of the population(s) you wish to sort?.To answer it we need to know the following information:
Guidelines for Getting a Good Sort - Sort Calulator (Designed by Larry Arnold)
#FLOWJO INTERNSHIP SOFTWARE#
The core reccommends the following software to aid you in your flow analysis, we also provide two offline workstations free of charge with most of this software installed:įor more information on FlowJo licencing please contact Kiran Gill or John Altman Yerkes Flow Core manages this, please direct questions to them. The flow core strongly reccommends that you use a Live/Dead marker in all of your panels. Proper Panel Design will also ensure you produce excellent data!.